HISTOPATHOLOGIC AND CYTOLOGIC TECHNIQUES

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Across
  1. 3. Requires 4 changes of wax at 15 minutes interval. May be completed in 1 hour.
  2. 5. A water soluble wax for enzyme histochemistry; does not require clearing and dehydration.
  3. 10. Cutting off excess paraffin wax to form a four-sided prism.
  4. 11. Removal of intercellular & extracellular water from tissues after fixation & prior to wax impregnation.
  5. 12. Composed of 2 or more simple components is combined.
  6. 14. Best decalcifying agent in electron microscopy and immunohistochemistry. Most common chelating agent in the market.
  7. 15. Mixture of highly purified paraffin and synthetic plastic polymers; melting point of 56-57 deg. celsius.
  8. 17. Usually contains glacia acetone acetic acid; for parts of cells.
  9. 18. A substitute for paraffin wax that used in infiltrating eye specimens.
  10. 21. Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.
  11. 22. Tissues should not be more than 4-5 mm thick except when processing lung specimens which require 2cm.
Down
  1. 1. Involves removal of clearing agent from tissues so that it will replace by a medium that will fill all cavities and tissues spaces.
  2. 2. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones.
  3. 4. It can dehydrate and clear tissues at the same time. May cause conjunctival irritation.
  4. 6. An automatic tissue processor that can do the first four steps in tissue processing, however it cannot perform decalcification.
  5. 7. It can act both as dehydrating agent and clearing agent, and is extremely dangerous and toxic.
  6. 8. A purified form of nictocellulose. For tissues with large and hollow and cavities that tends to collapse.
  7. 9. Similar to paraplast; melting point 56-58 deg. celsius.
  8. 13. Classified both as nuclear and histochemical fixative and it can preserve nuclear protein and mucopolysaccharide.
  9. 16. Highly flammable and carcinogenic, can damage BM leading to Aplastic anemia .
  10. 19. alcohol Recommended for plant and animal microtechniques.
  11. 20. A method recommended for urgent biopsies, for specimens with much air like lungs, brain, eyes, spleen and CNS.