Histopathologic and Cytologic Techniques

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Across
  1. 3. It fix bone marrow
  2. 4. It is excellent for glycogen demonstration
  3. 6. It fixes and precipitates nucleoproteins.
  4. 7. It is considered to be the most rapid fixative and may be used for urgent biopsy specimens for paraffin processing
  5. 8. Most common chrome osmium acetic acid fixative, excellent for nuclear structures
  6. 16. Similar to chloroform but cheaper
  7. 17. Also known as Ethylene Glycol monoethyl ether
  8. 23. It is not routine fixative and used for the precipitation of proteins and nucleic acids.
  9. 24. It may be use as holding solutions for tissues to be transported to frozen sections or kidney biopsies for special processing
  10. 26. Clearing agent for eye specimen
  11. 27. Suited for urgent biopsies
  12. 30. also known as Dioxane
  13. 31. Classified both as nuclear and histochemical fixative.
  14. 32. Also known as Gendre solution
  15. 33. A clearing agent recommended for clearing embryo, insect and other delicate specimen.
  16. 34. It is used for clearing, both for embedding and mounting procedures.
  17. 35. Tissues tend to become adulterated
Down
  1. 1. Defined as the killing, penetration and hardening of tissues
  2. 2. The removal of the dehydrating agent.
  3. 3. Recommended for fixation of embryos and pituitary biopsies and endometrial cuttings
  4. 5. A method that hard tissue must undergo
  5. 9. Most common metallic fixative
  6. 10. It fix myelin, peripheral nerve and also processing neurological tissues
  7. 11. Also known as formol sublimate
  8. 12. Recommended for preservation of enzyme & for fixed brain tissue for diagnosis of rabies
  9. 13. For early degenerative processes and tissue necrosis, rickettsia and other bacteria
  10. 14. Also known as Helly's fluid
  11. 15. Also known as Moller's fluid
  12. 18. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones
  13. 19. Recommended for enzyme histochemistry and EM
  14. 20. a dehydrating agent for section and smears
  15. 21. obtain in citrus fruit
  16. 22. Substitute for xylene and benzene
  17. 25. A clearing agent for cytological studies like smooth muscles of skin and for CNS tissue
  18. 28. Removal of intercellular & extracellular water from tissues after fixation & prior to wax impregnation
  19. 29. It is a fixation method for frozen specimen