HISTOPATHOLOGIC TECHNIQUES

2. Best decalcifying agent in electron microscopy and immunohistochemistry. Most common chelating agent in the market.
5. Mixture of highly purified paraffin and synthetic plastic polymers; melting point of 56-57 deg. celcius.
6. Requires 4 changes of wax at 15 minutes interval. May be completed in 1 hour.
8. Composed of 2 or more simple components is combined.
10. of intercellular & extracellular water from tissues after fixation & prior to wax impregnation.
11. Cutting off excess paraffin wax to form a four-sided prism.
12. An automatic tissue processor that can do the first four steps in tissue processing, however it cannot perform decalcification.
15. Tissues should not be more than 4-5 mm thick except when processing lung specimens which require 2cm.
16. Recommended for plant and animal microtechniques.
18. Involves removal of clearing agent from tissues so that it will replace by a medium that will fill all cavities and tissues spaces.
20. Similar to paraplast; melting point 56-58 deg. celsius.
21. A substitute for paraffin wax that used in infiltrating eye specimens.

1. Highly flammable and carcinogenic. Can damage BM leading to Aplastic anemia.
3. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones.
4. Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.
7. Usually contains glacia acetone acetic acid; for parts of cells.
9. A method recommended for urgent biopsies, for specimens with much air like lungs, brain, eyes, spleen and CNS.
13. A purified form of nictocellulose. For tissues with large and hollow and cavities that tends to collapse.
14. A water soluble wax for enzyme histochemistry; does not require clearing and dehydration.
17. It can act both as dehydrating agent and clearing agent, and is extremely dangerous and toxic.
19. Classified both as nuclear and histochemical fixative and it can preserve nuclear protein and mucopolysaccharide.