Across
- 3. It is a method of dioxane dehydration that 3 changes of pure dioxane followed by 3 changes of paraffin wax, embed the cool in water.
- 5. It is a routine clearing agent because of fast acting and with rapid clearing time 30 mins – hours AND NOT suited for nervous tissues and lymph nodes.
- 7. It is a weak and slow decalcifying agent.
- 8. It is a method that is immersing specimen in 4% aqueous phenol for 1-3 days.
- 11. It is not a routine fixative and most often used combination with other fixatives to produce compound fixative.
- 14. Removal of intercellular & extracellular water from tissues after fixation & prior to wax impregnation.
- 20. It is another form of celloidin soluble in equal concentration of ether and alcohol, with a lower viscosity.
- 23. is the process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities and give a firm consistency to the specimen.
- 24. are normally utilized for embedding celloidin blocks but are equally useful for paraffin wax blocks because of being cheap and easy to make.
- 27. It can act both as dehydrating and clearing agent and tissues tend to ribbon poorly.
- 29. It is a method of testing that is most reliable and accurate because it can detect even the smallest amount of calcium.
- 30. It is the removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.
- 35. It allows the general microscopic study of tissue structures without altering the structural pattern and normal intercellular relationship of tissues.
- 38. It can be used as ethanol substitute and used in microwave technique.
- 40. It is a useful compound for breaking down superfluous cell materials that would otherwise contaminate the nucleic acid sample.
- 43. It is a dehydrating agent that use for routine dehydration; Fast acting and non-toxic.
- 44. Is a process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is allowed to solidify.
- 45. It is a test that carried out by adding calcium carbonate.
- 46. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones
- 48. It is used to preserve chemical components of tissues like enzymes.
- 49. It contains combination of formaldehyde and mercuric chloride.
- 50. It is used as an embedding medium for delicate specimens and frozen tissue sections because it prevents fragmentation of tough and friable tissues when frozen sections are cut.
- 52. It is classified both as nuclear and histochemical fixative Preserve nuclear proteins and mucopolysaccharides.
- 53. it is an acid that is weak and slow; recommended only for teeth and small pieces of bones.
- 54. It is equipped with a warm plate to manage the impregnated specimen, and a cold plate at -5°C for rapid solidification of the block.
- 55. It is a volatile oil found in citrus fruit peels and can acts as a clearing agent.
- 57. It is a method of dioxane dehydration that the tissue wrap in a gauze bag and placed in a bottle with dioxane and anhydrous calcium oxide or quicklime.
- 58. is a product of paraffin, containing rubber, with the same property as Paraplast.
- 59. is a form of secondary fixation whereby a primarily fixed tissue is placed in aqueous solution of 2.5-3% potassium dichromate for 24 hours to act as mordant.
- 60. It involves in removal of clearing agent tissues that will be replaced by a medium that will fill cavities and tissue space.
Down
- 1. Most crucial and most important step in tissue processing.
- 2. It is combustible at 110-120°F and toxic by ingestion, inhalation and by skin contact by prolonged exposure.
- 4. It is a type of nitric acid that is considered as the most rapid.
- 6. It has a lower melting point (46-48°C), but it is harder than paraffin and not soluble in water, but is soluble in 95% Ethyl Alcohol and other clearing agents.
- 9. It is synthetic wax substitute similar to paraplast with a melting point of 56-58°C.
- 10. It can be a good substitute for cedarwood oil and an eyes specimen also it has a faint odor and with low evaporation rate.
- 12. It is extremely slow clearing agent for cytological studies like smooth muscles of skin and CNS Tissues.
- 13. It consist of 2 L- shaped strips of heavy brass or metal.
- 15. It is for rough tissues like skin, fibroid and decalcified tissues for nervous tissues, lymph nodes and embryos.
- 16. It is the process of cutting off excess paraffin wax to form a 4 sided prism.
- 17. A water soluble wax for enzyme and a technique suitable for many enzyme histochemical studies.
- 18. It is an aldehyde fixative that is recommended for enzyme histochemistry and Electron microscope.
- 19. is the simplest, most common and best embedding medium used for routine tissue processing.
- 21. It is embedding plastics are made up of a carefully balanced mixture of epoxy plastic, catalysts and accelerators.
- 22. It is suited for urgent biopsies and highly flammable and carcinogenic, can damage BM leading to aplastic anemia.
- 25. It is a mixture of highly purified paraffin and synthetic plastic polymers.
- 26. It is a dehydrating agent that is dissolves paraffin.
- 28. It is recommended for urgent biopsies, for specimens like lungs, brain, eyes, spleen and CNS.
- 31. It is recommended for clearing embryos, insects, and delicate specimen.
- 32. It is regarded as the most rapid fixative Recommended for fixing chromosomes and lymph glands.
- 33. is made up of a series of interlocking plates resting on a flat metal base, forming several compartments.
- 34. It is a fast acting fixative that supplied a 40% aqueous solution.
- 36. This reagent causes minimum shrinkage of tissues and tissues tend to become adulterated.
- 37. What specimen is recommended for 10% formalin.
- 39. A purified form of nitrocellulose that is soluble in alcohol and ether and for large and suitable for specimens with large hollow cavities which tend to collapse.
- 41. It is a substitute for xylene and benzene but it acidifies in a partially filled vessel.
- 42. It is a metallic fixative that may form black mercuric deposits remedy wash tissue with alcoholic iodine.
- 47. It can fix and dehydrate tissue at the same time and not commonly used because it evaporates easily and it is highly flammable.
- 51. It is a dehydrating agent that can dehydrate and clear tissue at the same time and toxic if ingested or inhaled.
- 56. A method That positively charged calcium ions are attracted to negative electrodes from the decalcifying solution thus facilitating calcium removal.