Across
- 2. Under negative atmospheric method inside the oven
- 4. Removal of clearing agent from tissues so that it will be replaced by a medium that will fill all cavities and tissue spaces
- 8. method in paraffin wax infiltration which uses autotechnicon
- 11. With rapid clearing time 30 mins – hours
- 14. tissue wrap in gauze bag and placed in a bottle with dioxane and anhydrous calcium oxide or quicklime
- 15. ____ ice trays needs to smear inner mold with glycerine to facilitate easy removal of blocks
- 19. ____ oil is used for clearing embryos, insects and other very delicate specimens
- 22. Traditionally, fixation is carried out at what temperature
- 25. Fixatives used to preserve chemical components of tissues
- 26. Recommended for fixing chromosome and lymph glands
- 29. we embed using paraffin wax
- 31. ____ FIXATIVES used for acid mucopolysaccharides and for tissue mucin
- 32. can be used as a decalcifying agent and as a tissue softener
- 36. for eyes; faint odor and has low evaporation rate; good substitute for cedarwood oil
- 37. Fixation ____ refers to the period the tissue is exposed to formalin
- 38. Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated
- 40. ____ away molds can produce perfect blocks even without trimming
- 41. Gross description is done by
- 42. Method in decalcification that is dependent on the supply of electric current
- 44. Most common chrome osmium acetic acid fixative, excellent for nuclear structures
- 47. a.k.a. mechanical method. Done by pricking, bending the tissue with needle
- 48. similar to paraplast; melting point of 56-58 degC
- 49. ____ colloidin uses Gilson’s Mixture combination of chloroform and cedarwood oil) for storage
- 50. ____ embedding when we infiltrate using celloidin
- 51. fast-acting fixative, smallest aldehyde fixative
- 53. Fixative that is not absorbed by tissues
- 55. Obtained form a citrus fruit
- 58. Substitute for xylene and benzene but not as fast
- 59. 3 changes of puredioxane followed by 3 changes of paraffin wax, embed the cool in water
- 60. dehydrating agent dissolves paraffin
- 61. a.k.a. X-ray. Considered as the most realiable/ accurate because it can detect the smallest amount of calcium
Down
- 1. With offensive odor, room should be well ventilated
- 3. It is used at ice cold temperatures -5 to 4°C
- 5. allows the general microscopic study of tissue structures without altering the structural pattern and normal intercellular relationship of tissues
- 6. Placing infiltrated tissue in a mold containing the embedding medium which is allowed to solidify
- 7. Highly flammable and carcinogenic, can damage bone marrow leading to aplastic anemia
- 9. a.k.a. diethylene dioxide
- 10. used in routine dehydrationbecause it is fast acting and non toxic
- 12. consist of 2 L- shaped strips of heavy brass or metal; prior to use, needs to be assembled; can produce block of different sizes
- 13. Recommended for touch preparation
- 16. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones
- 17. Method in fixation in which specimen is subjected to chemical fixatives
- 18. ____ Boat is easy to prepare; economical
- 20. Examples are Oleum ricini & castor oil
- 21. heat and ____ accelerates decalcification
- 23. with ____ oil, tissues tend to become adulterated
- 24. mixture of highly purified paraffin and synthetic plastic polymers
- 27. Defined as the killing, penetration and hardening of tissues.
- 28. Includes entering the details of the specimen in a log book
- 30. Toxic by ingestion, inhalation, and skin contact
- 33. method in paraffin wax infiltration which is carried out using oven
- 34. Classified both as nuclear and histochemical fixative because it can preserve mucopolysaccharide
- 35. used for infiltrating eye specimen
- 39. 2.5% solution is for small tissue fragments 4% is for larger tissues less than 4 mm thick
- 43. Citric acid citrate buffer: contains ____ as preservative
- 45. a.k.a. infiltration
- 46. Fixatives that are absorbed by tissues
- 52. ____ Colloidin uses 70% alcohol for storage
- 54. can be used as ethanol subtitute; (in clearing, substitute for xylene) used in microwave technique
- 56. solution that is recommended for fixation of embryos and pituitary biopsies and endometrial cuttings
- 57. a water soluble wax for enzyme histochemistry; no longer require dehydration and clearing.