Across
- 2. ________controls fluorescently tagged mouse antibodies to irrelevant antigens are used to assess non-specific binding to cells, helping to establish the threshold for positivity.
- 4. Change in wavelength when a fluorophore emits light
- 5. Confirming the accuracy of a selected gate by examining the expression of markers that should or shouldn't be present in that population.
- 6. T cell marker present on all mature T cells
- 9. Cluster of
- 10. Subdividing a gated population based on additional markers to provide more detailed characterization.
- 11. Used to set positivity in flow cytometry
- 12. ________ method: When the instrument's sample volume is precise, the cell count can be determined directly.
- 14. ________ analysis: Combining a typical cell counter with a flow cytometer to obtain both cell counts and percentages.
- 17. ________checks: Ensuring consistency between different measurements, like the sum of lymphocytes, monocytes, and granulocytes equaling the total WBC count.
Down
- 1. Using viability dyes to exclude dead cells and debris, ensuring the analysis focuses on live cell populations.
- 3. These cells have minimal side scatter in an automated cell counter
- 5. ________ method: Adding a known quantity of fluorescent beads to the sample and using their count to calculate the sample cell count.
- 7. A ______ cell sorter can collect specific cell populations
- 8. Cells must be in _____ _____ suspension for flow cytometry
- 13. Fluorescent molecules used to tag antibodies in flow cytometry
- 15. HIV virus depletes these cells.
- 16. Process in flow cytometry for selecting a population of cells to analyze