Across
- 3. The n-butanol pathway from this organism was inserted into S. cerevisiae
- 7. Patch density is defined as the ratio of this to cell area
- 8. This property was increased to improve tolerance to MCFAs (3 words)
- 10. Addition of 0.8% (v/v) n-butanol causes WT cells to arrest in this phase of the cell cycle (2 words)
- 12. This property was decreased to enhance alcohol tolerance (3 words)
- 13. This parameter is defined as the ratio of cable length to the distance between endpoints
- 14. This protein was used as a reporter for the repression unit
- 16. An advantage of engineering actin cables is less disruption to this cycle (3 words)
- 18. Addition of 0.2mM decanoic acid delayed formation of the endosome and this organelle
Down
- 1. Addition of 0.8% (v/v) n-butanol causes wild type yeast cells to adopt this type of morphology
- 2. Engineering actin patches has this advantage over engineering transcription factors (3 words)
- 4. Along with rational engineering, this strategy has previously been employed to increase yeast tolerance to alcohols (3 words)
- 5. This gene is responsible for the production of a-factor
- 6. Besides propidium iodide, this was used to stain dead cells
- 9. Engineering membrane transporters may not directly stabilize intracellular pH because of non-specific export of these (2 words)
- 11. The MCFA production strain was constructed by reinforcing the endogenous fatty acid pathway and engineering these (3 words)
- 15. In the n-butanol producing strain, alcohol dehydrogenase from this organism was integrated into the genome
- 17. Generally, this part of cells is prone to damage by biofuels