Lab Midterm Review

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Across
  1. 4. The ability of a lens to distinguish which are close together
  2. 6. stabilizes cells, enhances DNA binding, and reduces enzyme activity
  3. 8. What is the color of the spore stain
  4. 9. First stain used in gram stain
  5. 11. What does MAC stand for?
  6. 14. What are needles used for
  7. 16. Which technique uses a sterile swab (or sterile toothpick)
  8. 18. When bacteria take up naked DNA
  9. 19. What is the counter stain in the gram stain
Down
  1. 1. Washes away the primary dye in gram negative cells to pick up counter stain
  2. 2. What is the mordent in the gram stain
  3. 3. What color do the cells turn in the spore stain
  4. 5. What do you look through on a microscope
  5. 7. facilitates further interactions between primary dye and the cell wall. Crystallizes the dye that has penetrated the microbe.
  6. 10. refers to a small colony of bacteria that grows near a larger colony, often in the presence of an antibiotic.
  7. 12. Helps make bacteria competent to take up DNA
  8. 13. induces the expression of the GFP gene in the pGLO plasmid, allowing transformed E.coli to produce and fluoresce the green protein under UV light
  9. 15. What does MSA stand for?
  10. 17. increases membrane permeability and induces the uptake of plasmid DNA