Liquid Chromatography

6. decreasing the polarity of the mobile phase shortens the elution time for a ___ phase.
7. good separation method for rapid isolation and purification of biomolecules.
8. size exclusion chromatography is widely used to ___ molecular mass species.
9. refractive index method is a ___ detector for LC.
11. most sensitive detection method for LC.
12. elution mode where sample remains unchanged through he run.
14. bonded phase with intermediate polarity.
16. process in which removes air bubbles and breaks particles.
17. the plate is dried and rotated for ___ degrees in 2D TLC.
19. most polar solvent used as mobile phase in LC.
20. valve knob turns 60 degrees ___ from load position to inject position.

1. two reciprocated pumps with different phases are used to minimize the ___.
2. column with a similar phase as the main separation column.
3. for a 6-port LC injector, a ___ coil sample loop is used.
4. process in which fluorophores are introduced in sample molecule, increasing the analysis complexity.
5. the difference between the peak retention times divided by the average peak width.
10. ___ pump facilitates the movement of the mobile phase.
13. the smaller the packing particles, the ___ the retention time. Thus, the greater the plate number.
15. most common bonded phase to particles.
18. it uses adsorption for separation.