Liquid Chromatography

4. Decreasing the polarity of the mobile phase shortens the elution time for a ___ phase.
6. The smaller the packing particles, the ___ the retention time. Thus, the greater the plate number.
10. Process in which fluorophores are introduced in sample molecule, increasing the analysis complexity.
11. Process in which removes air bubbles and breaks particles.
14. Most sensitive detection method for LC.
17. Most polar solvent used as mobile phase in LC.
18. ___ pump facilitates the movement of the mobile phase.

1. Two reciprocated pumps with different phases are used to minimize the ___.
2. Column with a similar phase as the main separation column.
3. The difference between the peak retention times divided by the average peak width.
5. Good separation method for rapid isolation and purification of biomolecules.
6. For a 6-port LC injector, a ___ coil sample loop is used.
7. Valve knob turns 60 degrees ___ from load position to inject position.
8. The plate is dried and rotated for ___ degrees in 2D TLC.
9. Elution mode where sample remains unchanged through the run.
12. Most common bonded phase to particles.
13. Refractive index method is a ___ detector for LC.
15. Bonded phase with intermediate polarity.
16. Size exclusion chromatography is widely used to ___ molecular mass species.
19. It uses adsorption for separation.