Mammalian Cell Culture

2. Make free of ALL live bacteria
4. Growth of cells in an artificial environment outside of the organism
8. A cell-impermeable toadstool mushroom toxin that binds actin
9. Refers to coverage of the flask by the cells
11. Store stocks of cells to prevent the need to have cell lines in culture at all times
14. Etched glass chamber used to count cells

1. Distinguishing living cells from nonliving cells
2. the execution of cell culture procedures without introducing contaminating microorganisms from the environment
3. Green Fluorescent Protein detected by fluorescent microscopy
5. A nonionic detergent that disrupts cellular membranes without disturbing protein-protein interactions established by formalde-fresh
6. Crosslinks amino acids to stabilize the cell for examination
7. Breaks bonds between cells and lysine
10. Make free of MOST live bacteria
12. A DNA molecule used as a vehicle to transfer foreign genetic material into another cell
13. Artificial introduction of material into cells, typically involving the uptake of DNA
15. Fuse with Hela plasma membrane so that DNA is transported into the cell
16. Describes the behavior of cells in a fluid environment
17. Liquid designed to support growth of cells outside of the organism