Mammalian Cell Culture

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Across
  1. 2. Fuse with Hela plasma membrane so that DNA is transported into the cell
  2. 4. Breaks bonds between cells and lysine
  3. 5. Green Fluorescent Protein detected by fluorescent microscopy
  4. 10. Artificial introduction of material into cells, typically involving the uptake of DNA
  5. 13. Distinguishing living cells from nonliving cells
  6. 14. Make free of MOST live bacteria
  7. 15. Store stocks of cells to prevent the need to have cell lines in culture at all times
  8. 16. Etched glass chamber used to count cells
  9. 17. Growth of cells in an artificial environment outside of the organism
  10. 18. Liquid designed to support growth of cells outside of the organism
Down
  1. 1. Make free of ALL live bacteria
  2. 3. A cell-impermeable toadstool mushroom toxin that binds actin
  3. 6. Crosslinks amino acids to stabilize the cell for examination
  4. 7. Describes the behavior of cells in a fluid environment
  5. 8. Refers to coverage of the flask by the cells
  6. 9. the execution of cell culture procedures without introducing contaminating microorganisms from the environment
  7. 11. A DNA molecule used as a vehicle to transfer foreign genetic material into another cell
  8. 12. A nonionic detergent that disrupts cellular membranes without disturbing protein-protein interactions established by formalde-fresh