Manipulation Of Proteins

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Across
  1. 4. In gel filtration chromatography, the larger particles flow through (faster/slower)
  2. 6. The dye used in the Bradford assay
  3. 10. Enzyme used to lyse cells
  4. 12. Released by the formation of peptide bonds
  5. 14. Electrophoresis gel also known as the upper gel
  6. 16. All amino acids contain a carboxyl group and a/an _______ group
  7. 17. Electrophoresis gel that has a pH of 8.8
  8. 18. Non-specific protein purification
  9. 19. The sample is run ______ in a protein electrophoresis chamber
  10. 20. Buffer used to create high salt environment in the column
Down
  1. 1. Bonds that hold secondary structures together
  2. 2. Page pores are ________ compared to agarose
  3. 3. Assays used when no molar extinct coefficient is given
  4. 5. Interactions between _______ result in tertiary structure
  5. 7. Buffer used to create low salt environment in the column
  6. 8. Gel used to run protein electrophoresis
  7. 9. What type of structure is beta pleated sheets
  8. 11. Bonds that hold primary structure together
  9. 13. _______ and tryptophan are aromatic residues that absorb UV light at 280 nm
  10. 15. Negative charge binds to _____ amino acids during column chromatography