Microbiology Lab review

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Across
  1. 2. Which agar is selective for gram-negative rods and differential for lactose fermentation?
  2. 3. What is the name of the counterstain generally used for gram staining?
  3. 5. Term describing media of which exact chemical composition is unknown?
  4. 9. Which reagent is used in the citrate test?
  5. 10. What are chains of spherical cells called?
  6. 11. Which primary stain is used for acid fast staining?
  7. 13. What does breaking/cracking of the agar indicate?
  8. 14. What color do gram-positive cells stain?
  9. 16. Which organisms need oxygen because they cannot ferment or respire anaerobically and are poisoned by high concentrations of oxygen?
  10. 18. Which metabolic process is responsible for converting a carbohydrate, such as starch or a sugar, into an alcohol or acid?
  11. 20. The appearance of a positive Methyl red test is?
  12. 22. Is it better to use an older or younger organism for the endospore stain?
  13. 23. An IMVIC test that results in a ++-- is indicative of which organism?
  14. 24. What are pairs of rod shaped organisms called?
Down
  1. 1. Which stain is responsible for the color given to spores?
  2. 4. What is Kovac’s reagent used to identify in the SIM medium?
  3. 6. What is the most critical step when doing a gram stain?
  4. 7. Does TSI medium contain a lower concentration of glucose, sucrose or lactose?
  5. 8. What is the substrate for hydrogen sulfide production in the TSI agar?
  6. 12. Which organisms is Phenyl Ethyl Alcohol agar selective for?
  7. 15. What is the term for the cell surrounding the spore?
  8. 17. Which media ingredient supplies amino acids?
  9. 19. Which process kills the bacterial cells and causes them to adhere to the glass so that they do not get washed off during staining?
  10. 21. Is Phenyl Ethyl Alcohol agar selective or differential?