Module 1-3 GenPath, Histopath and Cytologic Techniques

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Across
  1. 5. the most common and the fastest decalcifying agent used so far
  2. 7. dehydrating agent is removed from the tissue and replaced with a substance that will dissolve the wax
  3. 8. fixative classified as both microanatomical and nuclear fixing agent
  4. 11. fixing agent that adulterates and denatures proteins by destroying hydrogen bonds and other bonds.
  5. 13. substances which combines with calcium ions and other salts to form weakly dissociated complexes and failitates the removal of calcium salts
  6. 15. hypertension & valve incompetence can lead to __________ hypertrophy
  7. 17. when using __________, the amount should be 5-10x the volume of the specimen
  8. 22. adequate water rinsing after decalcification can usually be accomplished for 30 minutes for __________ samples
  9. 25. fixative made up of two or more fixatives
  10. 27. agent can be used for both fixation and dehydration of urgent biopsies
  11. 30. first and most critical step in Histotechnology
  12. 31. schaudinn’s Ohlmacher’s and Carnoy –Lebrun solution are classified as __________ fixative
  13. 32. fixative is used for the precipitation of proteins and nucleic acids
  14. 37. obtained from citrus fruit; substitute for cedarwood oil
  15. 41. __________ organs should have cotton soaked in fixative or completely open
  16. 43. same properties with chloroform; cheaper but highly toxic
  17. 44. the chemical component becomes part of the tissue by forming cross-links or molecular complexes.
  18. 51. can be used an indicator for water saturation
  19. 52. decomposes upon exposure to sunlight
  20. 53. clearing agent that can be used as substitute for xylene and benzene
  21. 55. use of neutral __________ permits an excellent staining results
  22. 56. clearing agent can tissue/s become adulterated when immersed in it
  23. 57. group of fixatives can excellently preserve glycogen
  24. 60. use of __________ fixative can cause only minimal physical and chemical alteration of cells
  25. 61. tissues that are less than 3 mm thick can accelerate __________
  26. 62. __________ tissues may be washed out in running water overnight and immersed in 4% aqueous phenol solution
Down
  1. 1. general microscopic study of tissue structure and without alternating its structures
  2. 2. process of removing calcium or lime salts from the tissue
  3. 3. methyl benzoate, amyl acetate and methyl salicylate can be used to speed up dehydration and generally used for urgent __________ processing
  4. 4. the dehydrating agent recommended for plant and animal microtechniques
  5. 6. fixative that preserve chemical constituents of cells and tissues
  6. 7. can be both fixative and decalcifying agent; for decalcifying minute bone spicules
  7. 9. usable DNA fragments for PCR, poor fat solvent
  8. 10. fixative that preserve cytoplasmic structure
  9. 12. the only weak acid used extensively as a primary decalcifying agent
  10. 14. may be carried out in an already fixed tissue by it in an aqueous solution of 3% potassium dichromate for 24 hrs to act as mordant
  11. 16. test use to measure calcium carbonate
  12. 18. very weak decalcifying solution and is suitable only for minute pieces of bones
  13. 19. fixating agent is for connective tissue mucins and umbilical cord?
  14. 20. can be used as isopropylxylene substitute in rapid automated tissue processing
  15. 21. aside from clove oil, what other oil is unsuitable for routine clearing due to its expensiveness
  16. 23. involves slow substitution of the water in the tissue with an organic solvent
  17. 24. autopsy technique by __________ in which organs are removed one by one
  18. 26. reagent used to remove mercuric fixative
  19. 28. recommended dehydrant for sections and smears
  20. 29. may be used in staining series as dehydrant agent
  21. 33. the increase in breast size during puberty may be classified as __________ hypertrophy
  22. 34. fixative made up of only one component substance
  23. 35. accelerate staining and decalcification
  24. 36. most commonly used clearing agent
  25. 38. clearing agent that can be used for eye specimens
  26. 39. clearing agent for embryos, insects, and very delicate specimen
  27. 40. fixative capable of forming networks to allow rapid penetration
  28. 42. for routine processing, four changes of __________ are used
  29. 45. alteration in cell orientation is called __________
  30. 46. produces good nuclear staining
  31. 47. most ideal, sensitive and reliable test to measure completeness of decalcification
  32. 48. __________ fixation should be stretched for by sutures on each end
  33. 49. simple fixative for EM, histochemical and enzyme studies
  34. 50. most important in precipitation of nucleic acids from alcohol
  35. 52. fixative that preserve specific parts of the cell
  36. 54. carbon tetracholride has the same disadvantage as __________
  37. 58. other agent required when doing Weiseberger's method of dehydration aside from calcium oxide
  38. 59. fixative must be compatible with _____ to be used