MSc ELISA crossword

3. The term that describes antibodies that are identical, produced by a clone of a single antibody producing cell
7. The total number of wells of the 96-well plate you are using in your experiment today
8. What is measured at 450 nm as the outcome of the ELISA?
9. Target being detected
15. The “IS” of ELISA stands for
16. Running a sample in __________ is a standard scientific practice to improve the reliability of results by allowing for error detection and reducing variability.

1. The colour generated by the substrate, TMB in the ELISA
2. The method of dilution you used to prepare your sample standards
4. The animal that the capture antibody was produced in
5. Columns one, two and three are used for this ____________ curve
6. The ELISA format performed in this practical
10. The animal that the detection antibody was produced in
11. The step of the ELISA that prevents binding of protein to parts of the plate not occupied by the capture antibody
12. Used for detection/capture
13. In this experiment, your secondary antibody (the detection antibody) is conjugated to Horseradish Peroxidase (HRP); what is HRP?
14. Step to remove unbound components