PCR & DNA library

1. ________ is the genus of the thermostable DNA polymerase used in PCR.
5. ________ is an in-vitro, enzymatic amplification of a desired sequence of DNA using a pair of oligonucleotide primers.
6. ___________ are absent in cDNA library.
8. ______________ library produced from fully transcribed mRNA.
9. ______________ library represents complete genome in multiple clones containing small DNA fragments.
11. __________ is the third step in PCR where the new complementary strand is synthesized.
13. ________ is the species of the thermostable DNA polymerase used in PCR.
14. _________ is an important salt of PCR buffer which acts as a cofactor for DNA polymerase.
15. __________ DNA polymerase has proof-reading property.
16. ____________ is the first step in PCR where the target DNA strands are separated by heating to 940C
17. _________ elements are present with the fragments of clones of a genomic library.

2. __________ technique is used to detect RNA viruses and to study mRNA expression.
3. __________ PCR can be used to monitor the amplification of target and it can be quantified.
4. __________ is the second step in PCR where the primers bind to the ends of the target DNA at 45- 550C.
5. __________ are oligonucleotides of 18-22 nts which have complementary sequences of ends of target DNA.
7. __________ PCR is used to increase the specificity of the technique.
10. library __________ is a collection of independent clones of DNA which is also termed a clone bank.
12. _____________ of fragments of clones of cDNA library can easily be expressed.
14. __________ PCR is used to detect different targets in a single reaction.
18. _________ DNA polymerase is used in polymerase chain reaction.