PCR & DNA library

6. ___________ are absent in cDNA library.
7. _________ elements are present with the fragments of clones of a genomic library.
8. _________ is an important salt of PCR buffer which acts as a cofactor for DNA polymerase.
9. __________ DNA polymerase has proof-reading property.
10. __________ technique is used to detect RNA viruses and to study mRNA expression.
15. _____________ of fragments of clones of cDNA library can easily be expressed.
16. ________ is the species of the thermostable DNA polymerase used in PCR.
17. ________ is the genus of the thermostable DNA polymerase used in PCR.
18. _________ DNA polymerase is used in polymerase chain reaction.
19. __________ is the step in PCR where the new complementary strand is synthesized.
20. ______________ library produced from fully transcribed mRNA.

1. __________ is a collection of independent clones of DNA which is also termed a clone bank.
2. ________ is an in-vitro, enzymatic amplification of a desired sequence of DNA sequence.
3. ____________ is the step in PCR where the target DNA strands are separated by heating to 940C
4. ______________ library represents complete genome in multiple clones containing small DNA fragments.
5. __________ PCR is used to detect different targets in a single reaction.
11. __________ are oligonucleotides of 18-22 nts which have complementary sequences of ends of target DNA.
12. __________ PCR can be used to monitor the amplification of target and it can be quantified.
13. __________ PCR is used to increase the specificity of the technique.
14. __________ is the step in PCR where the primers bind to the ends of the target DNA.
15. _________ solution that is used to carryout PCR.