PLANAR CHROMATOGRAPHY

3. The process of removing compounds from a column using a solvent.
4. The force responsible for moving the solvent upward in TLC and paper chromatography.
7. Individual portions of eluate collected separately during column chromatography.
8. The most commonly used polar stationary phase in TLC.
9. The phase that remains fixed while analytes move through the system.
10. A chromatographic technique that separates molecules using highly specific biological interactions.
13. A chromatographic technique that separates compounds according to their electrical charge.
15. The liquid collected after passing through the chromatography column.
18. A locating reagent that detects primary amines by producing a pink color.
21. The ratio of the distance traveled by a solute to the distance traveled by the solvent front.
22. Poor flow caused by cracks or air pockets in a packed chromatography column.
23. A quantitative detection technique commonly used with HPTLC.

1. An advanced form of TLC that provides improved resolution and quantitative analysis.
2. A chromatographic technique that separates molecules according to their size and shape.
5. The solvent that transports analytes during chromatographic separation.
6. A general locating reagent that produces brown spots on a TLC plate.
10. The solid material that serves as the stationary phase in adsorption chromatography.
11. An adsorbent available in acidic, basic, and neutral forms.
12. The degree of attraction between a compound and a chromatographic phase.
14. The attachment of molecules onto the surface of the stationary phase.
15. The fresh solvent introduced into a chromatography column.
16. A laboratory technique used to separate the components of a mixture.
17. A chromatographic principle based on the distribution of compounds between two immiscible liquid phases.
19. A locating reagent used to detect sugars and compounds containing double bonds.
20. A locating reagent used to detect tertiary amines by producing an orange color.