Plant Biotechnology

2. Common carbon source in plant tissue culture.
5. Inventor of southern blotting.
6. Also known as Western Blotting.
8. Expansion of VNTRs
9. Used in sterilization.
11. A bacterium which lives in the boiling springs of Yellowstone National Park and similar habitats.
16. Genes responsible for tumor formation.
17. An alkaline solution to denature the double-stranded DNA.
18. A technique used to separate DNA/RNA fragments.
19. Technique used forpaternity and maternity testing; criminal identification and forensics
24. One type of the haploid culture .
26. Expansion of BSA.
27. One of the most widely used culture media developed by Murashige and Skoog.
29. Membrane for Western Blotting.
30. A technique developed in 1977 by James Alwine, David Kemp and George Stark at Stanford University.
32. English scientist invented RFLP in 1984.
33. Enzymes used to cut high-molecular-weight DNA strands into smaller fragments.
35. Enzyme used in PCR
37. An enzyme play an important role in plant tissue culture.

1. Single units of the bases A, T, G, and C.
3. Gel used for detection of proteins.
4. A complex sugar derived from seaweeds.
7. Widely used tissue culture technique to multiply orchids, other ornamentals, and fruit and forest tree species.
10. Expansions of IEDC.
12. Dye used to track DNA movement in a gel with blue colouration.
13. An online database publishing northern blots.
14. A triploid tissue.
15. Expansion of TBS.
20. Used in western blotting for Hybridization.
21. This help in acclimatization of successful transfer of the plantlets from laboratory plant field.
22. Used in buffer during Northern Blotting.
23. Stage II of Micropropagation.
24. This reaction occur at 54-60°C for 20-40 seconds in PCR.
25. Membrane used in blotting techniques
28. One of the stages of somatic embryogenesis.
31. This involves the use of a single arbitrary primer in a PCR reaction.
34. Used for staining fluoresces DNA in agarose.
36. Discovered polymerase chain reaction.