Across
- 4. the lower gel in SDS that has smaller pores
- 5. to provide appropriate pH
- 8. reaction where two molecules combine and release water molecule
- 9. the upper gel in SDS PAGE with a pH of 6.8
- 12. a non-specific type of protein purification
- 14. a brilliant blue dye used in SDS PAGE
- 16. short chain of amino acids linked by peptide bonds
- 19. rod-like secondary structure of proteins
- 21. hydrogen atom is shared between an oxygen or nitrogen
- 22. chromatography using antigens
- 23. strengthens filaments in muscles
Down
- 1. to mix a pellet back into a liquid
- 2. enzyme that breaks down bacterial cell walls
- 3. isolating a substance from other impurities
- 6. structure of protein where R-groups create ionic bonds
- 7. solid from a solution that isolates after centrifugation
- 10. structure where polypeptide chains form larger proteins
- 11. instrument where proteins are separated from a substance
- 13. part of a mixture that has been separated
- 14. carbon atom double-bonded to an oxygen and hydroxyl group
- 15. clear residue remaining after spinning a pellet
- 17. chromatography that separates proteins by charge
- 18. makes samples sink into wells for SDS PAGE
- 20. used to measure the concentration of total protein