Across
- 1. A method of protein purification in which molecules and proteins are separated by their affinity for specific molecules.
- 5. The process of removing the salt from a protein-salt solution.
- 6. A method of protein purification in which the proteins precipitate out of the solution at a certain salt concentration.
- 11. This method of protein separation is based on both the mass and isoelectric point of the protein.
- 13. A method of column chromatography in which molecules and proteins are separated by size.
- 15. This is the pH at which a molecule has a net charge of zero.
Down
- 2. A method of protein separation in which proteins are separated based on the pH at which they have a net charge of zero.
- 3. A method of protein purification in which molecules and proteins are separated by their net charge.
- 4. This is a technique used to separate DNA, RNA, and proteins and to check purification progress based on mass using an electric field.
- 7. A method used to separate different components and organelles in a homogenate based on their densities.
- 8. A test used at every step of the purification process to identify a protein using a specific property of that protein.
- 9. This technique used to check purification progress ensures that proteins are separated only based on molecular weight by denaturing all of the proteins and making them all have the same charge.
- 10. This is the mixture of the components and organelles of cells after the membranes of the cells have been removed.
- 12. A measurement used to determine the purity of a sample. It is calculated as the ratio of the activity of the enzyme to the amount of protein present in the assay.
- 14. A protein separation method that has a higher resolving power and separates molecules at a faster rate.