RECOMBINANT DNA TECHNOLOGY

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Across
  1. 3. major components are required in PCR
  2. 5. step in PCR consists of heating the reaction to 94-98°C for 20-30 seconds.
  3. 8. vector is including Plasmid, Bacteriophage, Cosmid and YAC (Yeast Artificial Chromosome).
  4. 9. is one of the application of the Recombinant DNA Technology.
  5. 10. has the biggest capacity to carry insertion size which is 100-1000 kilobases.
  6. 11. is a method that produces large quantities of specific segments in a test tube.
  7. 13. plants and animals are usually produced by the Recombinant DNA Technology.
  8. 14. has the least capacity to carry insertion size which is 0.1-10 kilobases.
  9. 15. DNA is the DNA containing gene of desired gene that will be inserted into the host cell to produced desired protein.
Down
  1. 1. DNA is DNA molecule made by combining genes from different organisms.
  2. 2. enzyme is an enzyme that catalyzes the joining of DNA fragments.
  3. 4. is an example for a heat resistance enzyme.
  4. 6. enzyme is the enzymes that recognizes and cut DNA into DNA fragments at specific base sequences (restriction site).
  5. 7. Transgenic, has desirable improved characteristics.
  6. 12. production using cDNA is also an application of The Recombinant DNA Technology.