RECOMBINANT DNA TECHNOLOGY

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Across
  1. 1. End is where both strands of the DNA molecule are cut at the same position, no nucleotides are left unpaired.
  2. 4. is an example of host cell for plasmid (Genetically Engineered).
  3. 6. plants and animals are usually produced by the Recombinant DNA Technology.
  4. 8. Transgenic, has desirable improved characteristics.
  5. 9. step in PCR consists of heating the reaction to 94-98°C for 20-30 seconds.
  6. 11. is a method that produces large quantities of specific segments in a test tube.
  7. 12. is an organism that had been used to receive a recombinant DNA for cloning purposes.
  8. 13. is an example for a heat resistance enzyme.
  9. 14. DNA is DNA molecule made by combining genes from different organisms.
  10. 15. vector is including Plasmid, Bacteriophage, Cosmid and YAC (Yeast Artificial Chromosome).
  11. 16. enzyme is an enzyme that catalyzes the joining of DNA fragments.
  12. 17. production using cDNA is also an application of The Recombinant DNA Technology.
  13. 19. has the biggest capacity to carry insertion size which is 100-1000 kilobases.
Down
  1. 2. DNA is the DNA containing gene of desired gene that will be inserted into the host cell to produced desired protein.
  2. 3. produced restriction enzyme.
  3. 5. is one of the application of the Recombinant DNA Technology.
  4. 7. is the production of exact copies (clones) of a particular gene.
  5. 10. enzyme is the enzymes that recognizes and cut DNA into DNA fragments at specific base sequences (restriction site).
  6. 11. has the least capacity to carry insertion size which is 0.1-10 kilobases.
  7. 18. End leaves one DNA strand longer than the other on each fragment of DNA.