RECOMBINANT DNA TECHNOLOGY

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Across
  1. 5. is an organism that had been used to receive a recombinant DNA for cloning purposes.
  2. 7. enzyme is the enzymes that recognizes and cut DNA into DNA fragments at specific base sequences (restriction site).
  3. 9. has the least capacity to carry insertion size which is 0.1-10 kilobases.
  4. 10. is a method that produces large quantities of specific segments in a test tube.
  5. 11. vector is including Plasmid, Bacteriophage, Cosmid and YAC (Yeast Artificial Chromosome).
  6. 12. is an example for a heat resistance enzyme.
  7. 14. enzyme is an enzyme that catalyzes the joining of DNA fragments.
  8. 15. has the biggest capacity to carry insertion size which is 100-1000 kilobases.
Down
  1. 1. produced restriction enzyme.
  2. 2. DNA is the DNA containing gene of desired gene that will be inserted into the host cell to produced desired protein.
  3. 3. is an example of host cell for plasmid (Genetically Engineered).
  4. 4. is the production of exact copies (clones) of a particular gene.
  5. 6. End is where both strands of the DNA molecule are cut at the same position, no nucleotides are left unpaired.
  6. 8. DNA is DNA molecule made by combining genes from different organisms.
  7. 13. End leaves one DNA strand longer than the other on each fragment of DNA.