RECOMBINANT DNA TECHNOLOGY

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Across
  1. 2. End is where both strands of the DNA molecule are cut at the same position, no nucleotides are left unpaired.
  2. 4. DNA is DNA molecule made by combining genes from different organisms.
  3. 6. is one of the application of the Recombinant DNA Technology.
  4. 8. Transgenic, has desirable improved characteristics.
  5. 10. is an example for a heat resistance enzyme.
  6. 13. plants and animals are usually produced by the Recombinant DNA Technology.
  7. 15. vector is including Plasmid, Bacteriophage, Cosmid and YAC (Yeast Artificial Chromosome).
  8. 17. is an organism that had been used to receive a recombinant DNA for cloning purposes.
  9. 18. is an example of host cell for plasmid (Genetically Engineered).
  10. 19. enzyme is an enzyme that catalyzes the joining of DNA fragments.
  11. 20. enzyme is the enzymes that recognizes and cut DNA into DNA fragments at specific base sequences (restriction site).
Down
  1. 1. production using cDNA is also an application of The Recombinant DNA Technology.
  2. 3. step in PCR consists of heating the reaction to 94-98°C for 20-30 seconds.
  3. 5. DNA is the DNA containing gene of desired gene that will be inserted into the host cell to produced desired protein.
  4. 7. produced restriction enzyme.
  5. 9. is the production of exact copies (clones) of a particular gene.
  6. 11. is a method that produces large quantities of specific segments in a test tube.
  7. 12. has the least capacity to carry insertion size which is 0.1-10 kilobases.
  8. 14. End leaves one DNA strand longer than the other on each fragment of DNA.
  9. 16. has the biggest capacity to carry insertion size which is 100-1000 kilobases.