RECOMBINANT DNA TECHNOLOGY

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Across
  1. 3. DNA fragments are cut out of their normal position in the chromosome using _______ enzymes.
  2. 5. proteins are widely used as _________ in laboratory experiments and to generate antibody probes for examining protein synthesis within cells and organisms.
  3. 8. based on autonomously replicating plasmids of Saccharomyces cerevisiae
  4. 10. group of individual cells or organisms descended from one progenitor
  5. 12. most common application of recombinant DNA
  6. 13. small replicating molecule in cloning is called a DNA _________.
  7. 16. vectors that are hybrids of plasmid and phage lambda
Down
  1. 1. DNA,DNA molecules formed by laboratory methods of genetic recombination
  2. 2. rice,A recombinant variety of rice that has been engineered to express the enzymes responsible for ß-carotene biosynthesis.
  3. 4. DNA is possible because DNA molecules from all organisms share the same ________ structure.
  4. 6. based on F-factor (fertility factor) plasmids of E. coli
  5. 7. library,contains DNA present in a given cell population which is prepared from the mRNA(messenger RNA) using the enzyme reverse transcriptase
  6. 9. are chosen depending on the _______ of DNA that must be included in a library.
  7. 11. a part of the main cellular genome,but they can carry genes that provide the host cell with useful properties and commonly found
  8. 14. is _________ from the organism under study and is cut into small fragments of a size for cloning.
  9. 15. library,a large collection of cloned DNA sequences from a single genome.