Recombinant DNA Technology

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Across
  1. 4. The gene of interest is inserted into the cut plasmid. DNA ligase is used to bind the sticky ends together.
  2. 5. Target DNA and plasmid are 'cut' at the specific site using the same restriction endonuclease.
  3. 7. Biotechnology also includes the modification of organisms or ___ products intended to improve the quality of human life.
  4. 9. Genetic engineering ___ DNA to allow individual gens tk be transferred from one organisms to another to produce recombinant DNA.
  5. 11. Recombinant DNA Technology is a study whereby researchers join together DNA from ___ organisms in the laboratory.
  6. 13. The transformed bacteria were ___ in a culture medium containing chloramphenicol.
  7. 14. Process of identifying the transformed bacteria which have successfully taken up the recombinant DNA.
Down
  1. 1. Small rings of DNA contained within some bacteria.
  2. 2. Step where the DNA is heated at 95°C for a few second.
  3. 3. DNA stands for ___ acid.
  4. 6. Biotechnology is the technology of applying living organisms or their ___ commercially in industries.
  5. 8. Target DNA and plasmid are isolated/extracted.
  6. 10. Step where the mixture is cooled to 55°C and incubated for a few minutes.
  7. 12. Recombinant DNA is whereby the donor's DNA ___ and the DNA of the recipient is joined together.
  8. 15. The recombinant DNA is transferred into a ___ form for gene expression.