RECOMBINANT DNA TECHNOLOGY

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Across
  1. 2. part of basic procedures in isolation
  2. 5. bacterial cell that allows the cloning vector to replicate within it
  3. 7. the desired cloned genes from the cloned cells
  4. 10. process which is a gene interest is found and copied out the extracted DNA from an organism
  5. 13. a techniques to rapidly replicate or ampify selected DNA segment that are initially present in extremely small amounts
  6. 14. of donor and plasmid DNA with the same restriction enzymes
  7. 16. are viruses which can insert their rDNA into bacteria for replication
  8. 17. steps in cloning
  9. 18. stage in PCR
  10. 19. of bacterial cell(reintroduction of recombinant DNA into plasmid).
Down
  1. 1. the gene to be cloned
  2. 3. sticky ends and blunt ends is two types of___________.
  3. 4. to carry the target gene into host cell
  4. 6. of DNA into vector
  5. 8. small and molecules within a cell that is physically separated from chromosomol DNA and can be replicate independently
  6. 9. modifying and recombining DNA to produce desired product
  7. 11. of vector and gene-source DNA
  8. 12. techniques and method used in understanding gene expression and gene manipulation
  9. 15. PCR is an__________process.