Recombinant DNA technology

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Across
  1. 1. Abbreviation for the sequence involved in the translation of foreign DNA which is present in an expression vector
  2. 3. rDNA technology, a ________ is a DNA used as a vehicle.
  3. 8. __________ is the production of exact copies (clones) of a gene by using recombinant DNA technique
  4. 10. A ____________ vector is used to translate the gene of interest to protein.
  5. 12. ________ treatment of competent cells allow the vector to enter into the host cells.
  6. 13. A ____________ vector is used only for inserting foreign genes / gene of interest
  7. 14. rDNA technology, most commonly used host cell is ___________
Down
  1. 1. DNA /_________ is an in vitro technique in which two different species of DNA are joined and introduced into a host for production of important biological such as vaccine.
  2. 2. ligase/To join the foreign DNA with the vector DNA, _________ enzyme is used
  3. 4. Competent cells are prepared by the use of chemical (chemical formula)
  4. 5. ____________ gene is responsible for blue/white colony selection.
  5. 6. ___________ is used to select recombinant vector containing host cells.
  6. 7. ___________ is the site of a vector where different RE sites are present for inserting foreign genes.
  7. 9. the preparation of rDNA, same _____________ enzyme is used to cut both the vector and gene of interest
  8. 11. First human recombinant protein produced was ____________ hormone.