Recombinant DNA technology

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Across
  1. 1. ___________ is the site of a vector where different RE sites are present for inserting foreign genes.
  2. 4. In_________rDNA technology, most commonly used host cell is ___________
  3. 6. ___________ is used to select recombinant vector containing host cells.
  4. 7. ligase/To join the foreign DNA with the vector DNA, _________ enzyme is used
  5. 9. First human recombinant protein produced was ____________ hormone.
  6. 10. Competent cells are prepared by the use of chemical (chemical formula)
  7. 11. __________ is the production of exact copies (clones) of a gene by using recombinant DNA technique
  8. 12. A ____________ vector is used to translate the gene of interest to protein.
  9. 13. rDNA technology, a ________ is a DNA used as a vehicle.
  10. 15. ________ treatment of competent cells allow the vector to enter into the host cells.
Down
  1. 2. A ____________ vector is used only for inserting foreign genes / gene of interest
  2. 3. DNA /_________ is an in vitro technique in which two different species of DNA are joined and introduced into a host for production of important biological such as vaccine.
  3. 5. ____________ gene is responsible for blue/white colony selection.
  4. 8. the preparation of rDNA, same _____________ enzyme is used to cut both the vector and gene of interest
  5. 14. Abbreviation for the sequence involved in the translation of foreign DNA which is present in an expression vector