Sectioning, Staining, and Mounting

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Across
  1. 5. Other name for rocking microtome
  2. 6. applied immediately to tissue after its removal from living body
  3. 8. water miscible preparation
  4. 11. Hightens the color of the dye
  5. 18. Involves application of different color to give contrast
  6. 20. Microtome for electron microscopy
  7. 21. Movable part is the knife
  8. 23. Best vital stain
  9. 25. Uses intermittent burst of carbon dioxide to freeze block holder and tissue
  10. 26. Tissue elements are demonstrated not by stains but by solutions of metallic salts
  11. 27. For mitochondria
  12. 28. Most dangerous microtome
  13. 29. Polishing and sharpening the cutting edge
  14. 32. Promote attachment of tissue sections to slides
  15. 33. Stains living cells
  16. 34. Invented sliding microtome
  17. 35. Hone that gives a more polishing effect
  18. 36. Tisse is first overstained
  19. 38. Microtome knive to cut paraffin
  20. 40. Device used to cut tissues
  21. 41. For fresh tissue microtomy
  22. 42. For electron microscope
  23. 44. Invented Rotary microtome
  24. 45. To prevent escape of fluid
  25. 46. Knife use to cut frozen sections
Down
  1. 1. Bridge/link between the dye and the tissue
  2. 2. Process of removing excess paraffin wax from tissues once properly fixed on the slide
  3. 3. Other term for decolorization
  4. 4. Less dangerous sliding microtome
  5. 7. Staining tissues with a color that is different
  6. 9. Invented freezing microtome
  7. 10. Invented rocking microtome
  8. 12. Process to remove nicks
  9. 13. Process of applying dye
  10. 14. A red acid dye for cytoplasmic stain
  11. 15. Cutting tissues to thin slices
  12. 16. Most common type of microtome
  13. 17. Stain in a definite sequence
  14. 19. Do not use in stroping
  15. 22. recommended for spx dehydrated and cleared with xylene
  16. 24. Selective removal of dye
  17. 30. Uses an accentuator or mordant
  18. 31. Trimming in semi-thin sectioning in EM
  19. 37. Most simple microtome
  20. 39. injected to any part of a living body
  21. 40. Applying a mounting medium
  22. 43. Staining technique that uses aqueous/alcoholic dye to produce a color