Across
- 1. Tennessee city on the Mississippi River
- 6. Twin Cities partner on the Mississippi River
- 9. First widely used antibiotic from a mold
- 12. Largest city by area in the contiguous United States
- 14. Florida Gulf Coast city on Hillsborough Bay
- 15. Mississippi coastal city paired with Biloxi
- 17. Biological preparation that confers immunity
- 22. Florida city crisscrossed by boating canals
- 25. Missouri city by the Gateway Arch on the Mississippi
- 27. Metro spanning Missouri and Kansas
- 28. Long-distance electrical communication using coded pulses
- 29. Largest city in Nebraska on the Missouri River
- 30. Eastern Idaho city by the Snake River
Down
- 1. Dits and dahs system for encoding characters
- 2. Coastal city west of Los Angeles
- 3. Louisiana city famous for jazz and Mardi Gras
- 4. Twin City in North Carolina with Moravian roots
- 5. Central Florida city known for theme parks
- 6. South Florida city famed for Art Deco and beaches
- 7. Sunshine City on Tampa Bay
- 8. Largest city in Montana on the Yellowstone River
- 10. South Texas city on a shallow bay
- 11. Industrial hub in the foothills of the Appalachians
- 13. Northwest Louisiana city on the Red River
- 16. Southern California city famous for a parade and bowl game
- 18. North Carolina city of the Research Triangle
- 19. North Carolina banking hub nicknamed the Queen City
- 20. Alabama port city on the Gulf Coast
- 21. Largest city in South Dakota on the Big Sioux River
- 23. Piedmont Triad city known for civil rights history
- 24. West Texas city on the Llano Estacado
- 26. Gateway to the Black Hills of South Dakota