Yeast Lab

3. Carbon source that causes the Htt gene to be expressed
6. What can be added to the end of a toxic Htt gene to relieve the toxicity?
10. Spectrophotometers are used to measure______ (2 words no space).
11. The most important reason why S. cerevisiae is used to model human diseases is because Yeast is a _____.
12. S. cerevisiae is a great ____ organism.
14. The most toxic form of the Htt gene
15. The toxicity of 103Q Htt gene is due to it forming protein___________?
18. Who is the best student?
19. The transformation method used in this lab (shortened)

1. The spot test is carried out to observe the potential ___ effect of the Huntington protein on the Yeast cells.
2. What type of disease does the Huntington gene cause in humans?
4. One of the reasons why the transformations may have failed is due to using a too hot _____.
5. What is removed from the media to select for the plasmid?
7. The most commonly used media when culturing Yeast
8. Carbon source most commonly used that does not cause Htt gene expression
9. The yeast strain used in this lab
13. What is the most common symptom of Huntington´s disease?
16. What is the selection marker used in the bacteria carrying the plasmid? (shortened)
17. Clean streaking is used to get ____ colonies.