Yeast Lab

4. The most important reason why S. cerevisiae is used to model human diseases is because Yeast is a _____.
10. What type of disease does the Huntington gene cause in humans?
12. What is the selection marker used in the bacteria carrying the plasmid? (shortened)
15. S. cerevisiae is a great ____ organism.
16. Spectrophotometers are used to measure______ (2 words no space).
17. The most toxic form of the Htt gene.

1. Carbon source that causes the Htt gene to be expressed
2. What can be added to the end of a toxic Htt gene to relieve the toxicity?
3. Clean streaking is used to get ____colonies.
5. What is removed from the media to select for the plasmid?
6. The toxicity of 103Q Htt gene is due to it forming protein___________?
7. The most commonly used media when culturing Yeast.
8. Carbon source most commonly used that does not cause Htt gene expression.
9. The transformation method used in this lab(shortened).
11. What is the most common symptom of Huntington´s disease?
13. The spot test is carried out to observe the potential ___ effect of the Huntington protein on the Yeast cells.
14. The yeast strain used in this lab.