Crossword Puzzle

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Across
  1. 3. Acts as a decalcifying acid and tissue softener
  2. 5. Routine clearing agent with rapid clearing time of 30 mins – hours
  3. 6. Recommended for fixation of embryos and pituitary biopsies
  4. 7. Autopsy technique which involves "en block"
  5. 9. A necrosis where the affected organ appears "soft and greasy"
  6. 14. Complete non-appearance of an organ, absence of organ
  7. 16. Often used as a criterion for malignancy
  8. 17. Paraffin wax infiltration that can be used in infiltrating eye specimens
  9. 21. Made up of acrylic or methacrylic acid
  10. 22. Method of celloidin which infiltrates bone, brains, teeth and whole organs
  11. 27. It is a paraffin wax infiltration under negative atmospheric pressure inside the oven
  12. 28. It evaporates easily and highly flammable
  13. 29. Recommended for Mayling's specimen and for colored tissue photography
  14. 33. Due to the transfer of internal heat to the surface or site of injury
  15. 35. With anhydrous sodium acetate; for preserving bone marrow
  16. 37. It happens when the cell DNA and proteins are damaged beyond repair
  17. 39. A cytological method in which cells are dislodged by various tools from body surfaces
  18. 42. A clearing agent for eyes with a faint odor and low evaporation rate
  19. 43. An embedding where we infiltrate using celloidin then embedding using paraffin wax
  20. 44. Optimal temperature for decalcification
  21. 45. Known to be the smallest aldehyde fixative
  22. 48. Purplish discoloration of the skin due to sinking of fluid blood into capillaries of the independent part of the body
  23. 50. The most important requirement before doing the autopsy
  24. 52. Placing infiltrated tissue in a mold containing the embedding medium
  25. 55. It contains potassium dichromate and 40% formaldehyde
  26. 57. Carried out using paraffin wax infiltration and need to have an oven
  27. 58. Dissolves paraffin
  28. 59. Not a routine clearing agent and toxic to the liver
Down
  1. 1. To remove clearing agent from tissues and fill up cavities and tissue space
  2. 2. Developed at National Cancer Institute in December 1988
  3. 4. Similar to paraplast with a melting point of 56-58°C
  4. 6. Prolonged exposure of this clearing agent can damage the bone marrow
  5. 8. One of the most important processes in which the pathologist arrives at a diagnosis
  6. 10. Recommended for enzyme histochemistry and EM
  7. 11. Study of all changes that underlie a disease
  8. 12. Excellent for glycogen demonstration
  9. 13. Alteration of tissues by stabilizing protein so that tissues become resistant
  10. 15. A pattern of cell death that is accidental and unregulated
  11. 18. Used for acid mucopolysaccharide
  12. 19. It can be used as ethanol substitute and used in microwave technique
  13. 20. Mechanism of action which when used combines with the tissue
  14. 23. A method that is carried out by adding calcium carbonate
  15. 24. A purified form of nitrocellulose and also called as Celloidon Infiltration Method
  16. 25. Also known as ethylene glycol monoethyl ether
  17. 26. Can be used to preserve nuclear proteins and mucopolysaccharide
  18. 30. Rapidly denatures and precipitates proteins
  19. 31. Routine dehydrating agent that is fast-acting and non-toxic
  20. 32. Best decalcifying agent in EM and immunohistochemistry
  21. 34. A modern method of identification
  22. 36. The starting point in every disease
  23. 38. Regarded as the most rapid fixative
  24. 40. Slow and partly because the epoxy plastic itself is a large molecule
  25. 41. For Rickettsia and other bacteria
  26. 46. Its purpose is to detect malignancies and cancerous conditions
  27. 47. Removal of water from tissues after fixation and prior to wax impregnation
  28. 49. Method of celloidin which infiltrates eye specimen
  29. 51. Cutting off excess paraffin wax to form a four-sided prism
  30. 53. Recommended for plant and animal microtechniques
  31. 54. Performed to determine the events leading to the death of the patients
  32. 56. Consist of 2 L- shaped strips of heavy brass or metal