Across
- 4. Factor influencing electroendosmosis flow?
- 7. The overall speed of protein migration in native PAGE depends on size and this property
- 9. Dye used in electrophoresis to monitor sample migration
- 11. the typical shape of double stranded DNA molecules, which affects how they move through gel pores
- 14. Stain used to visualize proteins after native PAGE
- 15. The direction of fluid is towards?
- 16. The gel layer responsible for separating protein by size
- 17. this fluorescent dye binds between the stacked base pairs of DNA, making it visible under ultraviolet light
- 19. a buffer not to be used for proteins
- 21. A method of PAGE that denatures proteins, unlike native PAGE
- 22. proteins if globular, then surface area is?
- 23. This enzyme is commonly used to cleave DNA into fragments for electrophoresis
Down
- 1. The gel commonly used in PAGE to separate proteins
- 2. Migration of charged particles in medium requires?
- 3. The Fragment of DNA with a known sequence used as a standard in electrophoresis
- 5. The buffer system often used to maintain protein structure during native PAGE
- 6. Reducing agent that breaks disulfide bonds in proteins
- 8. suppose there are 3 types of DNA- ssDNA ,dsDNA and ds plasmid and you are to carry out electrophoresis, which of these will have faster electrophoretic mobility?
- 10. Proteins can be separated using electrophoresis based on?
- 12. Gel component with lower acrylamide concentration for protein separation
- 13. poor medium causes
- 15. Used to form loading wells in a gel, removed after the gel has set
- 18. Type of material exhibiting electroendosmosis
- 19. Gradient formed in isoelectric focusing to separate protein
- 20. the parameter that determines the degree of ionization of organic compounds
