Across
- 3. An automatic tissue processor that can do the first four steps in tissue processing, however it cannot perform decalcification.
- 6. Cutting off excess paraffin wax to form a four-sided prism.
- 8. of intercellular & extracellular water from tissues after fixation & prior to wax impregnation.
- 10. Removal of dehydrating agent from tissues to replaced it with a medium that will dissolve the wax with which the tissues is to be impregnated.
- 11. Mixture of highly purified paraffin and synthetic plastic polymers; melting point of 56-57 deg. celcius.
- 13. Usually contains glacia acetone acetic acid; for parts of cells.
- 15. Classified both as nuclear and histochemical fixative and it can preserve nuclear protein and mucopolysaccharide.
Down
- 1. Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones.
- 2. A method recommended for urgent biopsies, for specimens with much air like lungs, brain, eyes, spleen and CNS.
- 4. Composed of 2 or more simple components is combined.
- 5. It can act both as dehydrating agent and clearing agent, and is extremely dangerous and toxic.
- 7. Involves removal of clearing agent from tissues so that it will replace by a medium that will fill all cavities and tissues spaces.
- 9. Requires 4 changes of wax at 15 minutes interval. May be completed in 1 hour.
- 12. Tissues should not be more than 4-5 mm thick except when processing lung specimens which require 2cm.
- 14. Highly flammable and carcinogenic. Can damage BM leading to Aplastic anemia.