Histopathology (Decalcification, Infiltration, Mounting and Embedding)

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Across
  1. 2. Disposable embedding mold used in ordinary refrigerators and recommended for busy routine laboratories
  2. 5. Example of aqueous mounting media which may also be used as preservative, and with a high index of refraction
  3. 6. decalcifying agent with decalcifying time of 1-3 days and should be used in fume hood
  4. 7. Involves the wax impregnation under negative atmospheric pressure
  5. 10. Decalcifying agent with Chloroform as preservative
  6. 13. procedure whereby calcium or lime salts are removed from tissue following fixation
  7. 15. Medium for impregnation used for specimens with large hollow cavities which tend to collapse
  8. 16. Celloidin method for processing whole eye sections
  9. 18. decalcifying agent which decalcifies and softens the tissue at the same time
  10. 21. simplest,most comon and best embedding medium used forroutine tissue processing
  11. 22. synthetic wax recommended for embedding eyes
  12. 23. Embedingg plastic miscible with acetone
  13. 24. Technique whereby positively charged calcium ions are attracted to a negative electrode then removed
  14. 26. hydrochloric acid with Sodium chloride
  15. 29. Mounting Medium recommended for mounting frozen sections from water
Down
  1. 1. recomended for whole mounts and for thick sections because it does not shrink much
  2. 3. form of celloidin which requires plasticizers and is more explosive
  3. 4. Polyethyleneglycol for many enzyme histochemical and cytologic studies
  4. 8. Used as a fixative and decalcifying agent
  5. 9. Blocking-out mold model with Equipped with a warm and cold plate
  6. 10. Made up of series of interlocking plates resting on a flat metal base, forming several compartments
  7. 11. Ammonium form of polystrene resin
  8. 12. Consist of two L-shaped strips of heavy brass or metal arranged on a flat metal plate
  9. 14. Most common chelating agent in the market
  10. 17. Other term with glycerol-based epoxy plastic
  11. 19. Process by which a tissue is arranged in precise position in the mold during embedding
  12. 20. mounting medium used for methlyene blue-stained nerve preparations
  13. 25. Disposable embedding mold utilize for embedding celloidin blocks. They also have advantage of being cheap and easy to make
  14. 27. most common and fastest decalcifying agent used
  15. 28. For routine decalcification of postmortem research tissues