Recombinant DNA Technology

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Across
  1. 1. use a DNA sequence to find complementary DNA or RNA sequences in a gel via hybridization.
  2. 3. a type of blot that uses a give antibody to identify proteins of interest.
  3. 7. identify the specific nucleotide sequence of a piece of DNA
  4. 9. AKA restriction enzyme, naturally occurring enzyme that cuts up DNA sequences at specific regions called restriction sites.
  5. 13. DNA molecules that accept foreign DNA to be replicated
  6. 16. the cell that replicates the vector and foreign DNA
  7. 17. creates a map of restriction sites.
  8. 18. making identical copies of a specific DNA molecule
Down
  1. 2. Basic local alignment search tool
  2. 4. 1. source of DNA 2. Endonuclease/restriction enzyme 3. Vector 4. Host Cell
  3. 5. a type of library that preserves the entire genome of an organism.
  4. 6. collections of recombinant phage or bacteria that each contain a different cloned piece of DNA from the same organism.
  5. 8. DNA created by joining together pieces of DNA from different sources
  6. 10. a type of blot that uses a DNA sequence to identify complementary RNA sequences of interest.
  7. 11. primers that are single stranded DNA about 10-20 bases long; act as a starting piece for the replication of the strand in PCR.
  8. 12. a type of blot that uses a DNA sequence to identify complementary DNA sequences of interest.
  9. 14. any DNA or RNA sequence that is complementary to some part of a cloned sequence present in the library' has a label or tag so it can be identified when it finds the match.
  10. 15. a type of library where the DNA sequences that are preserved in the bacteria/phage, are complementary to the mRNA that was being produced in a cell when the sample was taken.